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英文名稱:HK;Hexokinase from Saccharomyces cerevisiae;ATP:D-Hexose-6-phosphotransferase;Hexokinase from yeast
其他名稱:己糖磷酸激酶
CAS號(hào):9001-51-8
級(jí)別:BR
類型:Type F-300
分子量:~ 54 kDa (monomer);~110 kDa (dimer)
活力:≥130 units/mg protein (biuret)
活力定義:One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25℃
Optimal pH:7.5~9.0
Extinction Coefficient:E1% = 8.85 (PI) and 9.47 (PII) at 280 nm
Anion traces:sulfate (SO42-):free
Foreign activity:ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01%
性狀:白色鱗狀粉末。釀酒酵母提取。催化劑(Activators):Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds;抑制劑(Inhibitors):sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
用途:生化研究。為催化己糖6位上由ATP使之產(chǎn)生磷酸化反應(yīng)的酶。對(duì)α-D葡萄糖的親和性很高(Km=1×10-5M),對(duì)其他的己糖也可進(jìn)行磷酸化。此反應(yīng)的平衡向右偏移?!鱃°’=4.0千卡。該酶可被Mg2 、 K 所活化,但可被HS試劑阻抑。
保存:-20℃
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